Evaluation of canine epidermis and keratinocytoids (keratinocytic organoids) by transmission electron microscopy

BACKGROUND: Skin disease is a common complaint in veterinary medicine. Current models often use live animals. Epidermal organoids (keratinocytoids) are 3D miniature organs created in culture from single epidermal keratinocytes. These keratinocytoids can be used as an alternative to live animal studies to investigate epidermal structures and skin diseases. HYPOTHESIS/OBJECTIVE: This study's objective was to compare ultrastructural morphological features of canine keratinocytoids to those features of normal epidermis. RESULTS: Skin and organoids had morphologically similar components, including tight junctions, desmosomes, lamellar bodies and keratin filaments. These structures were similar in both morphology and distribution. Keratinocytes in the organoids had larger and more distinct keratohyalin granules than epidermal keratinocytes and contained small amounts of glycogen. Keratinocytes from the skin showed no glycogen accumulation. CONCLUSIONS AND CLINICAL RELEVANCE: Canine keratinocytoids are a useful model to study canine epidermal disease from a light microscopy and ultrastructural standpoint.

Authors: C. C. Lui, D. J. Wiener, K. R. Groch, L. W. Stranahan, C. R. Heard and A. G. Armién Title: Evaluation of canine epidermis and keratinocytoids (keratinocytic organoids) by transmission electron microscopy Full source: Vet Dermatol, 2025,Vol Document type: Journal Article
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Abstract	Source
BACKGROUND: Skin disease is a common complaint in veterinary medicine. Current models often use live animals. Epidermal organoids (keratinocytoids) are 3D miniature organs created in culture from single epidermal keratinocytes. These keratinocytoids can be used as an alternative to live animal studies to investigate epidermal structures and skin diseases. HYPOTHESIS/OBJECTIVE: This study's objective was to compare ultrastructural morphological features of canine keratinocytoids to those features of normal epidermis. RESULTS: Skin and organoids had morphologically similar components, including tight junctions, desmosomes, lamellar bodies and keratin filaments. These structures were similar in both morphology and distribution. Keratinocytes in the organoids had larger and more distinct keratohyalin granules than epidermal keratinocytes and contained small amounts of glycogen. Keratinocytes from the skin showed no glycogen accumulation. CONCLUSIONS AND CLINICAL RELEVANCE: Canine keratinocytoids are a useful model to study canine epidermal disease from a light microscopy and ultrastructural standpoint.